Skip to main content
  • Home
  • About
  • Faculty Experts
  • For The Media
  • Videos
  • Topics
    • Alumni
    • Events
    • Faculty
    • Library
    • Research
    • Students
    • All Topics
  • Contact
  • Submit
STEM
  • All News
  • Arts & Culture
  • Business & Economy
  • Campus & Community
  • Health & Society
  • Media, Law & Policy
  • STEM
  • Veterans
  • |
  • Alumni
  • The Peel
  • Athletics
Sections
  • All News
  • Arts & Culture
  • Business & Economy
  • Campus & Community
  • Health & Society
  • Media, Law & Policy
  • STEM
  • Veterans
  • |
  • Alumni
  • The Peel
  • Athletics
  • Home
  • About
  • Faculty Experts
  • For The Media
  • Videos
  • Topics
    • Alumni
    • Events
    • Faculty
    • Library
    • Research
    • Students
    • All Topics
  • Contact
  • Submit
STEM

Scientist Awarded Grants from ALS, Oak Ridge Associated Universities

Friday, July 8, 2016, By Rob Enslin
Share
College of Arts and Sciencesresearch

A researcher in the College of Arts and Sciences has been awarded grants from The ALS Association and Oak Ridge Associated Universities (ORAU) to support his study of protein biosynthesis.

nerve cells

Nerve cells, or neurons, found in the nervous system

Carlos A. Castañeda, assistant professor of biology and chemistry, is the University’s inaugural recipient of a one-year, $50,000 starter grant from the ALS Association, a nonprofit organization that seeks to find a cure for Amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig’s Disease. ALS is a progressive neurodegenerative disease that affects nerve cells in the brain and spinal cord. Castañeda will use the award to investigate the effects of a protein called Ubiliquin-2 (UBQLN2) on ALS.

Castañeda also is the recipient of ORAU’s 2016 Ralph E. Power Junior Faculty Enhancement Award, whose $5,000 prize is being matched by the University. He will use the grant to study Ufm1, a protein that regulates the cellular stress response of the endoplasmic reticulum (ER), and is linked to diabetes, migraine, breast cancer and other diseases. ORAU is a national consortium of more than 100 major institutions, committed to groundbreaking research in science, education, security and health.

“We are extremely proud of Professor Castañeda,” says Ramesh Raina, associate professor and chair of biology. “He is one of the field’s rising stars who is using his talent, curiosity and knowledge to meet future needs in critical areas, including human health. With these awards come prestige and value, both of which reflect favorably upon him and the life sciences at Syracuse.”

UBQLN2 is a protein-encoding gene whose mutations are commonly associated with ALS and dementia. Castañeda says that UBQLN2 interacts with both poly-ubiquitin chains, which mark cellular proteins for degradation, and proteasomes, which function like cellular recycling machines, breaking down proteins into smaller peptides and amino acids for reuse.

“We believe that a dysfunction of UBQLN2 is relevant for ALS pathology,” says Castañeda, whose research involves proteins associated with neurodegenerative and neuromuscular diseases. “We’re focusing on a particular stretch of amino acids [in UBQLN2] that is affiliated with ALS, and plan to characterize it, structurally and dynamically.”

Carlos Castañeda

Carlos Castañeda

Castañeda and his colleagues hope to prove that the sequence in question interacts with other ALS-relevant proteins, such as TDP-43, an RNA-binding protein; UBXD8, an intrinsic membrane protein; and hnRNPA1, another RNA-binding protein, which is commonly found in breast cancer cells.

The regulation of cellular levels of certain proteins—a process critical to cell survival and viability—is governed by protein synthesis and degradation. Proteins slated for degradation are usually tagged by a small signaling protein called ubiquitin. UBQLN2 then directs the unneeded or damaged proteins to the proteasome, where they are destroyed or recycled.

Unique to UBQLN2 is a stretch of prolines, which are a special class of structurally rigid amino acids. Castañeda contends that ALS-related mutations to prolines affect the structure and dynamics of UBQLN2, thus hindering the degradation process.

“For instance, we know that UBQLN2 interacts with TPD-43, whose dysregulation is found in 97 percent of all ALS cases,” Castañeda says. “By determining where the interaction takes place on each protein, we can better understand the role of UBQLN2 in ALS, and create a foundation for new druggable therapies involving it and possibly TDP-43.”

Castañeda’s work for ORAU involves a step after protein biosynthesis known as post-translational modification (PTM), in which a long chain of amino acids undergoes various transformations that confer new functional properties on the protein. He is using his ORAU award to study the cellular role of a new PTM called Ufm1 and its effect on protein structure, dynamics and function.

Like ubiquitin (a small protein that signals the degradation of proteins), Ufm1 has numerous biological responsibilities. Chief among them is regulating the ER—a network of membranes in a cell that manufactures and packages protein.

Working with Jon Madison, a geneticist at the Broad Institute of MIT and Harvard, Castañeda will spend the next year investigating the biochemical and biophysical possibilities of poly-Ufm1 chains. The Syracuse professor is optimistic that the project will generate sufficient preliminary data to garner larger, federal support.

“Our understanding of how Ufm1 functions is limited, due to the inability to make poly-Ufm1 chains,” says Castañeda, one of 35 ORAU award recipients. “We hope to make Ufm1 signals, using a hybrid chemical and enzyme approach. This way, we can study the signal itself, to see how it interacts with target proteins.”

If successful, the project will be the first to identify specific proteins that interact with poly-Ufm1 chains.

Castañeda is also intrigued by the fact that Ufm1 is found in neurons. “It may have a role in mental illness, such as schizophrenia,” he says. “Our lab will use various experimental and computational methods [including biomolecular nuclear magnetic resonance] to probe this protein on a molecular level.”

Castañeda joined the Syracuse faculty in 2014, following a postdoctoral fellowship at the University of Maryland at College Park. (He earned a Ph.D. from Johns Hopkins University.) In addition to PTMs, he studies structural biology and protein dynamics. His research has been supported by the National Science Foundation, the American Chemical Society, the Burroughs Wellcome Fund and, more recently, the Nappi Family Research competition.

Other recent ORAU award recipients from Syracuse include chemistry professors Tara Kahan and Ivan Korendovych.

  • Author

Rob Enslin

  • Recent
  • Activities for the Weekend of April 22-25
    Thursday, April 22, 2021, By News Staff
  • Annual Remembrance and Lockerbie Scholar Convocation to Be Held April 30
    Thursday, April 22, 2021, By News Staff
  • Watch Pomp and Ceremony of the 104th Chancellor’s Review Award Ceremony Live on April 23
    Wednesday, April 21, 2021, By Brandon Dyer
  • Chancellor Syverud Provides Updates to the University Senate
    Wednesday, April 21, 2021, By News Staff
  • Let’s Talk About Current Issues Welcomes Open Dialogue
    Wednesday, April 21, 2021, By Gabrielle Lake

More In STEM

Intelligence++ Competition Winners Announced

Three interdisciplinary teams won the inaugural Intelligence++ design and entrepreneurship competition held at Syracuse University Libraries’ Blackstone LaunchPad & Techstars (LaunchPad) on April 16. MeetCute won the $15,000 gold prize, HG Sensory won the $10,000 silver prize and Fundwurx won…

Fusion of Art and Science Leads to Discovery

Robert Wysocki arrived at Syracuse University in 2008, having made a name in the art world by capturing landscapes in three dimensions. Known for large sand sculptures showcased in galleries from Los Angeles to Florida, Wysocki’s inspiration began on a…

Bioengineering Ph.D. Student Receives National Recognition for Breakthrough Molecular Computational Tool

Nandhini Rajagopal’s accomplishments are massive even though her research focuses on small molecules. As part of biomedical and chemical engineering Professor Shikha Nangia’s research group, the Ph.D. student has focused her work on minute interactions between protein molecules in the…

New Study From Department of Biology Highlights Ways to Support Students in Virtual Learning Environments

The mass migration to virtual learning that resulted from the COVID-19 pandemic led to a profound change in student learning. While it presented many challenges, it also created opportunities for documenting responses. Two researchers from the Department of Biology in…

Research Computing: A Decade of Discovery on Campus

Do you need more computing power to move your work forward? Since 2011, the Research Computing team within Information Technology Services (ITS) has helped faculty and staff tackle computational challenges beyond the capabilities of a normal desktop or laptop computer. Each…

Subscribe to SU Today

If you need help with your subscription, contact sunews@syr.edu.

Connect With Us

  • Twitter
  • Facebook
  • Instagram
  • Youtube
  • LinkedIn
Social Media Directory

For the Media

Find an Expert Follow @SyracuseUNews
  • Facebook
  • Instagram
  • Youtube
  • LinkedIn
  • @SyracuseU
  • @SyracuseUNews
  • @SUCampus
  • Social Media Directory
  • Accessibility
  • Privacy
  • Campus Status
  • Syracuse.edu
© 2021 Syracuse University News. All Rights Reserved.